TABLE 2.
Comparison between fragment sizes (in numbers of bases) revealed by DNA sequencing and capillary electrophoresis
| Allele | Size obtained by sequencinga | No. of repeats | Size obtained by capillary electrophoresis from indicated source (difference from size obtained by sequencing)
|
Difference between sizes obtained from indicated sources
|
||||
|---|---|---|---|---|---|---|---|---|
| de Valke | Warwick | Imperial | de Valke vs Warwick | de Valke vs Imperial | Warwick vs Imperial | |||
| STRAf 3A | 158 | 17 | 159.7 (+1.7) | 159.3 (+1.3) | 157.1 (−0.9) | −0.4 | −2.6 | −2.2 |
| 164 | 19 | 166.5 (+2.5) | 165.7 (+1.7) | 163.6 (−0.4) | −0.8 | −2.9 | −2.1 | |
| 173 | 22 | 175.3 (+2.3) | 175.0 (+2.0) | 172.6 (−0.4) | −0.3 | −2.7 | −2.4 | |
| 185 | 26 | 187.8 (+2.8) | 187.2 (+2.2) | 184.7 (−0.3) | −0.6 | −3.1 | −2.5 | |
| 224 | 39 | 228.4 (+4.4) | 226.3 (+2.3) | 223.4 (−0.6) | −2.1 | −5.0 | −2.9 | |
| 233 | 42 | 236.7d (+3.7) | 235.1 (+2.1) | 232.7 (−0.3) | −1.6 | −4.0 | −2.4 | |
| 242 | 45 | 246.8d (+4.8) | 244.7 (+2.7) | 241.7 (−0.3) | −2.1 | −5.1 | −3.0 | |
| 245 | 46c | 249.8 (+4.8) | 247.4 (+2.4) | 244.7 (−0.3) | −2.4 | −5.1 | −2.7 | |
| STRAf 3B | 162 | 9 | 160.5 (−1.5) | 160.1 (−1.9) | 159.2 (−2.8) | −0.4 | −1.3 | −0.9 |
| 165 | 10 | 163.5 (−1.5) | 163.1 (−1.9) | 162.1 (−2.9) | −0.4 | −1.4 | −1.0 | |
| 168 | 11 | 166.5 (−1.5) | 165.9 (−2.1) | 165.2 (−2.8) | −0.6 | −1.3 | −0.7 | |
| 195 | 20 | 192.6d (−2.4) | 191.6 (−3.4) | 191.3 (−3.7) | −1.0 | −1.3 | −0.3 | |
| 201 | 22 | 198.0 (−3.0) | 197.1 (−3.9) | 196.9 (−4.1) | −0.9 | −1.1 | −0.2 | |
| 240 | 35 | 235.0d (−5.0) | 233.7 (−6.3) | 234.2 (−5.8) | −1.3 | −0.8 | +0.5 | |
| STRAf 3C | 96 | 11 | 95.2d (−0.8) | 94.3 (−1.7) | 92.4 (−3.6) | −0.9 | −2.8 | −1.9 |
| 114 | 17 | 114.0 (0.0) | 112.5 (−1.5) | 111.1 (−2.9) | −1.5 | −2.9 | −1.4 | |
| 117 | 18 | 117.2 (+0.2) | 115.6 (−1.4) | 114.0 (−3.0) | −1.6 | −3.2 | −1.6 | |
| 120 | 19 | 120.5 (+0.5) | 118.4 (−1.6) | 117.1 (−2.9) | −2.1 | −3.4 | −1.3 | |
| 132 | 23c | 132.5d (+0.5) | 130.7 (−1.3) | 129.5 (−2.5) | −1.8 | −3.0 | −1.2 | |
| 135 | 24 | 135.7 (+0.7) | 134.0 (−1.0) | 132.6 (−2.4) | −1.7 | −3.1 | −1.4 | |
| 141 | 26 | 141.9d (+0.9) | 140.3 (−0.7) | 138.9 (−2.1) | −1.6 | −3.0 | −1.4 | |
| 153 | 30 | 154.4d (+1.4) | 154.0 (+1.0) | 152.1 (−0.9) | −0.4 | −2.3 | −1.9 | |
| 165 | 34 | 166.9 (+1.9) | 166.3 (+1.3) | 165.0 (0.0) | −0.6 | −1.9 | −1.3 | |
| 207 | 48 | 209.9d (+2.9) | 208.4 (+1.4) | 207.1 (+0.1) | −1.5 | −2.8 | −1.3 | |
| STRAf 4A | 178 | 8 | 180.6 (+2.6) | 180.2 (+2.2) | 178.3 (+0.3) | −0.4 | −2.3 | −1.9 |
| 186 | 10 | 188.9 (+2.9) | 188.3 (+2.3) | 186.2 (+0.2) | −0.6 | −2.7 | −2.1 | |
| 190 | 11c | 192.9d (+2.9) | 192.2 (+2.2) | 190.0 (−0.0) | −0.7 | −2.9 | −2.2 | |
| 194 | 12 | 197.4 (+3.4) | 196.4 (+2.4) | 194.1 (+0.1) | −1.0 | −3.3 | −2.3 | |
| 202 | 14 | 205.4d (+3.4) | 204.3 (+2.3) | 201.9 (−0.1) | −1.1 | −3.5 | −2.4 | |
| 206 | 15 | 209.6 (+3.6) | 208.2 (+2.2) | 206.0 (0.0) | −1.4 | −3.6 | −2.2 | |
| 209b | 16 | 212.7d (+3.7) | 211.4 (+2.4) | 209.0 (0.0) | −1.3 | −3.7 | −2.4 | |
| 213b | 17 | 216.7 (+3.7) | 215.2 (+2.2) | 213.1 (+0.1) | −1.5 | −3.6 | −2.1 | |
| 222 | 19 | 226.3 (+4.3) | 224.7 (+2.7) | 222.0 (0.0) | −1.6 | −4.3 | −2.7 | |
| STRAf 4B | 166 | 5 | 166.7 (+0.7) | 166.0 (0.0) | 164.3 (−1.7) | −0.7 | −2.4 | −1.7 |
| 178 | 8 | 179.4 (+1.4) | 178.2 (+0.2) | 176.3 (−1.7) | −1.2 | −3.1 | −1.9 | |
| 182 | 9 | 183.0 (+1.0) | 182.3 (+0.3) | 180.5 (−1.5) | −0.7 | −2.5 | −1.8 | |
| 186 | 10c | 187.6 (+1.6) | 186.2 (+0.2) | 184.4 (−1.6) | −1.4 | −3.2 | −1.8 | |
| 190 | 11 | 191.6 (+1.6) | 190.4 (+0.4) | 188.3 (−1.7) | −1.2 | −3.3 | −2.1 | |
| STRAf 4C | 163 | 5 | 164.0 (+1.0) | 163.8 (+0.8) | 163.0 (0.0) | −0.2 | −1.0 | −0.8 |
| 171 | 7 | 172.5 (+1.5) | 172.2 (+1.2) | 170.6 (−0.4) | −0.3 | −1.9 | −1.6 | |
| 175 | 8c | 176.4 (+1.4) | 176.2 (+1.2) | 174.7 (−0.3) | −0.2 | −1.7 | −1.5 | |
| 267 | 31 | 270.9d (+3.9) | 269.3 (+2.3) | 267.2 (+0.2) | −1.6 | −3.7 | −2.1 | |