TABLE 2.

Genes used for MLST

Gene	Gene product	Primer (direction, sequence [5′-3′])a	Amplicon size (bp)	Sequence start	Sequence end	Gene length (bp)	No. of SNPs	No. of genotypes	No. of genotypes/site
ADE2	Phosphoribosylamino-imidazole carboxylase	Fwd, GTCACTTCTCAGTTTGAAGC	600	AAACAAAT	CTCATTTA	470	9	21	2.33
Rev, ACACCATCTAAAGTAGAGCC
HIS3	Imidazole glycerol phosphate dehydratase	Fwd, GGAGGGGACATATCACTGCC	534	AATCCCAA	GTTGATTG	400	8	14	1.75
Rev, AATCTTTAATTGCCAAAGCC
LEU2	Isopropyl malate dehydrogenase	Fwd, CTGTGAGACCAGAACAGGGG	802	GTAACTTT	AAGCTCTC	619	9	17	1.89
Rev, GCAGAGCCACCCAAGTCTCC
LYS2	l-Aminoadipatesemialdehyde dehydrogenase	Fwd, ATCTGAGAAGCAGTTGGCGC	631	AAAGATTG	TCTGAACT	441	10	19	1.90
Rev, AGACTTGTAAGAATTATCCC
NMT1	N-Myristoyltransferase	Fwd, CTGATGAAGAAATCACCG	738	GCTTCATTT	GAAGTGAA	537	11	22	2.00
Rev, GCTTGATATCATCTTTGTCC
TRP1	Phosphoribosylanthranilate isomerase	Fwd, AGCTATGTCGAGCAAAGAGG	503	ATATGAGG	CAGGTGGG	380	11	22	2.00
		Rev, ACATCAACGCCACAACACCC

^aFwd, forward; Rev, reverse.