TABLE 2.
Pyrazinamide susceptibility testing with the BACTEC 460TB system and pncA DNA sequencing analysis results for 412 non-M. canettii isolates of the Mycobacterium tuberculosis complexa
| No. of strains | Identification | BACTEC 460TB result | pncA mutation | Nucleotide change |
|---|---|---|---|---|
| 166 | MTB | S | No mutation | NA |
| 42 | MTB | S | Thr47Ala | A139G |
| 2 | MTB | S | Ser65Ser* | C195T |
| 1 | MTB | S | Ala20Ala* | G60A |
| 24 | AFR | S | No mutation | NA |
| 4 | MICR | S | No mutation | NA |
| 1 | CAP | S | No mutation | NA |
| 14 | MTB | R | Frameshift mutation | Deletion of nucleotide 70 (G) |
| 13 | MTB | R | Leu85Pro | T254C |
| 6 | MTB | R | NA | A→−11 upstream |
| 3 | MTB | R | Ser104Arg | C312G |
| 3 | MTB | R | Leu172Pro | T515C |
| 2 | MTB | R | Gln10Pro | A39C |
| 2 | MTB | R | Cys14Arg and Ser65Ser* | T40C and C195G |
| 2 | MTB | R | His51Gln | C153A |
| 2 | MTB | R | Leu116Arg | T347G |
| 2 | MTB | R | Trp119Cys | G357T |
| 2 | MTB | R | Thr142Pro and Ser65Ser* | A424C and C195T |
| 2 | MTB | R | Frameshift mutation | Nucleotide insertion (G) between codons 77 and 78 |
| 1 | MTB | R | NA | Deletion of start codon and −5 nucleotides upstream |
| 1 | MTB | R | Val21Gly | T62G |
| 1 | MTB | R | Leu35Arg and His82Asp | T104 and C244G |
| 1 | MTB | R | Ala46Val | C138T |
| 1 | MTB | R | Frameshift mutation | Deletion of nucleotide 146 (A) |
| 1 | MTB | R | His51Arg | A152G |
| 1 | MTB | R | Ser67Pro | T199C |
| 1 | MTB | R | Trp68Arg | T202C |
| 1 | MTB | R | His71Arg | A212G |
| 1 | MTB | R | Gly78Cys | G232T |
| 1 | MTB | R | Tyr103Asp | T307G |
| 1 | MTB | R | Gly108Arg | G322C |
| 1 | MTB | R | Frameshift mutation | Nucleotide insertion (G) between codons 104 and 105 |
| 1 | MTB | R | Frameshift mutation | Deletion of codons 128, 129, 130 |
| 1 | MTB | R | Val128Gly | T383G |
| 1 | MTB | R | Frameshift mutation | Nucleotide insertion (GG) between codons 130 and 131 |
| 1 | MTB | R | Cys138Tyr | G413A |
| 1 | MTB | R | Val139Leu | G415C |
| 1 | MTB | R | Val139Ala | T416C |
| 1 | MTB | R | Gln141Stop | C421T |
| 1 | MTB | R | Thr142Met | C425T |
| 1 | MTB | R | Frameshift mutation | Nucleotide insertion (CT) between codons 155 and 156 |
| 1 | MTB | R | Leu156Pro | T467C |
| 1 | MTB | R | Gly162Ser | G485A |
| 1 | MTB | R | Gly162Asp | G486A |
| 1 | MTB | R | Ser164Pro and Glu173Glu* | T490C and G519A |
| 1 | MTB | R | Ala171Val | C512T |
| 1 | MTB | R | Frameshift mutation | Nucleotide insertion (CG) at codon 177 |
| 1 | MTB | R | Val180Phe | G538T |
| 1 | MTB | R | Frameshift mutation | Deletion of four nucleotides (542, 543, 544, 545) |
| 45 | BOV | R | His57Asp | C169G |
| 43 | BCG | R | His57Asp | C169G |
| 1 | CAP | R† | No mutation | NA |
a
Abbreviations and symbols: MTB, M. tuberculosis; BOV, M. bovis; AFR, M. africanum; CAP, M. caprae; MICR, M. microti; S, susceptible; R, resistant; NA, not applicable; *, silent mutation; †, a live culture could not be retrieved to confirm the accuracy of the discrepant result (M. caprae strains are naturally PZA susceptible).