TABLE 1.
Comparison of the sensitivities of five PCR methods for the detection of G. duodenalis trophozoite DNA and cysts in stool specimens
| Primers | Target gene locus | Expected amplicon size (bp) | Reference | PCR resulta for:
|
|
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Trophozoite DNA concn (pg/μl) of:
|
Cyst concn (cysts/ml) of:
|
||||||||||||
| 100,000 | 10,000 | 1,000 | 100 | 10 | 1 | 3,368 | 674 | 337 | 168 | ||||
| RH11/RH4 and GiarF/GiarR | SSU rRNA | 130 | 3 | + | + | + | + | + | − | + | + | + | + |
| G7/G759 and forward/reverse | β-Giardin | 511 | 2 | + | + | + | + | − | − | − | − | − | − |
| GDHeF/GDHiR and GDHiF | Glutamate dehydrogenase | 432 | 9 | + | + | + | − | − | − | + | + | + | − |
| AL3543/AL3546 and AL3544/AL3545 | Triosephosphate isomerase | 530 | 11 | + | + | + | − | − | − | + | − | − | − |
| EF1AR/GLONGF and RTef1-αF/RTef1−αR | Elongation factor 1-alpha | 191 | 6 | + | + | − | − | − | − | ||||
a
All PCR amplifications were done in triplicate. +, at least one positive result out of three attempts.