TABLE 2.

Characteristics of loci used for MLVA

Locus	Repeat size (bp)	Repeat no. in straina:		No. of variantsb	Nei's diversity indexc	Coding region
		LK5	PT4
SE-1	7	4.9	4.9	6	0.62	None identified
SE-2	7	4.7	5.7	8	0.73	None identified
SE-3	12	4.0	3.0	3d	0.54	None identified
SE-4	117	2.7	2.7	3	0.05	valU
SE-5	6	NF	11.8	11	0.77	yohM
SE-6	33	10.8	10.8	4	0.07	bigA
SE-7	61	8.5	8.5	7d	0.60	ygbF
SE-8e	87	1	1	2	0.45	None identified
SE-9	9	2.1	3.1	4	0.53	ushA
SE-10	45	3.9f	8.1	2	0.05	tolA

^aRepeat number was determined from the genomic sequence using TRF, which identified partial repeats in flanking sequences. Inspection of loci SE-2 and SE-9 in the sequences of PT4 and LK5 revealed that they vary by one whole repeat unit. NF indicates that the sequence was not found in the incomplete sequence of LK5.

^bNumber of different fragment size polymorphisms detected among 156 Salmonella serotype Enteritidis isolates tested, including outbreak and sporadic isolates.

^c1 − ∑(variant frequency). See footnote b.

^dIncludes a null variant (no fragment amplified by PCR).

^eLocus SE-8 was identified by RAPD marker analysis and was found to be variable in strains of Salmonella serotype Enteritidis other than LK5 and PT4.

^fPartial sequence.