FIG. 1.

M. genitalium strain R6G grown in Vero cell culture from a urethral swab specimen from a Danish man was added to urine or SP4 mycoplasma medium kept at room temperature or at 4°C. At 1, 2, 4, 7, 14, and 21 days of incubation, cells from 1.9 ml of urine or SP4 mycoplasma medium were harvested and frozen at −80°C. Each specimen was inoculated into Vero cell cultures, and growth was monitored by quantitative PCR. (A and B) R6G in urine at 4°C (A) and room temperature (B). (C) R6G in SP4 medium at room temperature.