TABLE 2.

Inhibition rates of selective antibiotics at different concentrations on a selection of M. genitalium strains^a

Strain	% Inhibition at antibiotic concnb:
	Ampicillin (μg/ml)			Penicillin G (IU/ml)			Amphotericin B (μg/ml)			Polymyxin B (μg/ml)
	500	250	125	500	250	125	25	12.5	6.3	500	250	125
G37T	63.2	6.2	9.7	0	20.0	4.5	44.6	0	0	29.1	2.7	NTc
M30	67.1	23.0	41.7	44.2	25.5	12.5	95.2	13.4	17.1	79.9	53.6	NT
M2282	51.8	3.18	NT	27.6	0	NT	0	0	NT	0	0	NT
M2300	64.9	33.6	32.7	33.6	12.4	18.0	65.3	10.0	8.8	31.0	19.2	NT
M2321	33.1	21.8	0	3.3	0	0	6.0	7.8	23.3	55.0	67.0	NT
M2341	94.6	31.6	68.4	42.0	59.9	21.8	39.5	47.4	10.1	62.3	44.3	NT
M6090	76.1	0	0	0	0	0	95.5	0	0	0	0	NT
M6151	69.3	61.6	26.8	69.6	48.4	0	100	0	0	29.5	0	NT
R5G (M6280)	96.7	25.8	0	42.3	46.7	35.9	100	67.3	63.9	70.2	58.3	NT
R6G (M6281)	99.7	92.1	0	99.5	91.8	0	99.9	62.4	35.4	19.5	24.0	0
R65G (M6285)	99.9	79.6	37.8	99.1	7.1	16.4	100	72.5	0	83.0	0	NT
R66G (M6328)	99.9	96.9	94.3	99.9	56.9	28.3	99.9	99.9	76.9	98.2	0	NT
R67G (M6286)	99.4	95.8	73.2	94.9	0	0	99.5	71.0	0	0	0	NT

^aM. genitalium strains were grown in Vero cell suspensions with selective antibiotics at different concentrations in wells of flat-bottom tissue culture plates at 37°C in 5% CO₂. The M. genitalium DNA load in the supernatant was determined by quantitative real-time PCR 21 days after the inoculation of the M. genitalium strains.

^bThe inhibition rate was calculated by the following formula: inhibition rate (%) = [(average of DNA loads in control wells − DNA loads in test well)/(average of DNA loads in control wells)] × 100.

^cNT, not tested.