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. 2006 Nov 22;45(3):752–761. doi: 10.1128/JCM.01683-06

FIG. 2.

FIG. 2.

Detection density plot of LDR-FMA products. White areas represent expected result targets given the microspheres specified. Classification 1 and classification 2 each represent the signal intensity from one of two fluorochromes applied to Luminex FlexMAP polymer microspheres. Dots within white areas represent individual detection events as determined by the BioPlex suspension array system, where allele-specific LDR products are labeled with both “classification” (the fluorescent microsphere at the 5′ end) and “reporter” (R-phycoerythrin at the 3′ end) fluorescent signals. Individual dhps alleles are identified by Arabic numerals above the target area as follows: 1, 436S-437A; 2, 436S-437G; 3, 436A-437G; 4, 436F-437G; 5, 540K; 6, 540E; 7, 581A; 8, 581G; 9, 613A; 10, 613S. Individual dhfr alleles are identified by lowercase letters at the upper right edge of the target area as follows: a, 51N; b, 51I; c, 59C; d, 59R; e, 108S; f, 108N; g, 108T; h, 164I; i, 164L. Individual pfcrt alleles are identified by Roman numerals below the target area as follows: I, CVMNK; II, SVMNT; III, CVIET. Analysis was performed using BioPlex Manager (version 3.0) software.