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X-NO in wild-type YHB1 and mutant yhb1 cells after DETA NONOate treatment. Mid-log phase cells were cultured aerobically in the presence of 3 mM DETA NONOate at 30°C for 1 h. High-mass NO complexes (X-NO) in cell extracts were measured and normalized against protein content. Data (means ± SEM) were from three independent experiments.
