Figure 4.

Hypersensitivity of yhb1 mutants to growth inhibition by DETA NONOate but not H₂O₂ in room air. Mid-log phase YHB1 wild-type Y190 (open) and isogenic yhb1 mutant (filled) cells were cultured in YPD supplemented, as shown in A, with 0 (circles), 1 (triangles), and 3 (squares) mM DETA NONOate and, as shown in B, with 0 (circles), 0.2 (triangles), and 0.6 (squares) mM H₂O₂. Data shown are representative of two similar experiments. (C) Haploid JK93d and derivative yhb1 clones were cultured in the presence of 0 (open) and 3 mM (filled) DETA NONOate for 20 h. Shown is growth (relative fold increase in cell numbers compared with numbers at time 0; means ± SD; n = 8).