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. 2000 Apr 11;97(9):4672–4676. doi: 10.1073/pnas.090083597

Figure 5.

Figure 5

Anaerobic protection against DETA NONOate by YHB1. Yeast cells were cultured in a glove box for 5 days; studies were initiated on day 3. (A) Wild-type Y190 (open) and isogenic yhb1 (filled) cells were cultured in YPD supplemented with 0 (circles), 100 μM (triangles), 200 μM (diamonds), and 400 μM (squares) DETA NONOate. Data are the means of two independent experiments. (B) Aerobic NO clearance by 100 μg of lysates of anaerobically cultured yhb1 (thin solid line) or YHB1 (thick solid line) cells or 20 μg of lysates of aerobically cultured YHB1 (dotted line) cells.