TABLE 2.

Pyrosequencing and VITEK 2 results of the alpha-hemolytic blood culture isolates

Group and no.	Pyrosequencing resulta		VITEK 2 result		Unresolved (n)d
	Species	Scoreb	Species	Confidence levelc (n)
S. anginosus group
7	S. anginosus	100	S. anginosus	Exc (4)
			S. anginosus or S. gordonii	LD (2)
			Inconclusive identification	— (1)	1
3	S. anginosus	96.9	S. anginosus	Exc (1)
			S. anginosus or S. gordonii	LD (2)
6	S. intermedius	100	S. intermedius	Exc (4)
			S. intermedius or S. constellatus subsp. pharyngis	LD (1)
			S. constellatus subsp. pharyngis	Good (1)
3	S. constellatus	100	Inconclusive identification	— (2)	2
			S. pyogenes, S. constellatus subsp. constellatus, or S. dysgalactiae subsp. equisimilis	LD (1)
2	S. constellatus or S. intermedius	96.9	S. constellatus subsp. constellatus	Exc (1)
			S. constellatus subsp. constellatus, S. gordonii, or S. anginosus	LD (1)	1
S. sanguinis group
7	S. sanguinis	100	S. sanguinis	Exc (6) or Good (1)
2	S. sanguinis	96.9	S. sanguinis	Exc (2)
5	S. sanguinis	93.7	S. mitis/S. oralis	Exc (2), VG (1), or Good (1)	4
			S. mitis/S. oralis or S. constellatus subsp. pharyngis	LD (1)	1
4	S. sanguinis	90.5	S. mitis/S. oralis	Exc (2), VG (1), or Good (1)	4
1	S. gordonii	100	S. gordonii	Exc (1)
1	S. gordonii	87.4	S. gordonii	Exc (1)
S. salivarius group
5	S. salivarius or S. vestibularis	100	S. salivarius	Exc (3)
			S. infantarius	VG (1)	1
			Unidentified organism	— (1)	1
2	S. salivarius or S. vestibularis	96.9	S. salivarius	Exc (2)
S. mitis group
4	S. mitis	100	S. mitis/S. oralis	Exc (1), VG (1), Good (1), or Acc (1)
1	S. oralis	100	S. mitis/S. oralis	Exc (1)
24	S. mitis, S. pneumoniae, or S. pseudopneumoniae	96.9	S. mitis/S. oralis	Exc (10), VG (5), or Good (3)
			S. pluranimalium or S. mitis/S. oralis	LD (1)	1
			S. mitis/S. oralis or S. constellatus susbp. pharyngis	LD (1)
			Unidentified organism	— (4)	4
6	S. infantis	93.7	S. mitis/S. oralis	Exc (2), VG (2), or Good (1)
			Unidentified organism	— (1)	1
1	S. oralis	93.7	S. mitis/S. oralis	VG (1)
1	S. oralis	90.5	S. mitis/S. oralis	Exc (1)
1	S. peroris	93.7	S. mitis/S. oralis	Exc (1)
3	S. mitis or S. oralis (v2)e	100	S. mitis/S. oralis	Exc (1) VG (1), Good (1)
S. bovis group
2	S. gallolyticus subsp. gallolyticus	100	S. gallolyticus	Exc (2)
2	S. bovis or S. lutetiensis	100	S. infantarius	VG (1), Exc (1)
1	S. gallolyticus subsp. pasteurinanus	100	S. pasteurianus	Exc (1)
2	S. bovis or S. lutetiensis	96.9	S. infantarius	Exc (1)
			S. gallolyticus, S. lutetiensis/bovis or S. hyointestinalis	LD (1)
Undefined group
2	S. oralis or S. gallinaceus (v1)e	100	S. parasanguinis	VG (1)	1
			S. parasanguinis or S. mitis/S. oralis	LD (1)	1
1	Both regions heterogeneousf		S. mitis/S. oralis	Exc (1)	1

^aThe isolates have been listed according to the pyrosequencing result.

^bThe score is a measure of the relatedness of the studied sequence to the type strain sequence determined by the Identifire software. Score 100 is given to sequences that are identical to each other.

^cConfidence level is a measure of the strength of the VITEK analysis in the descending order Exc (excellent), VG (very good), good, Acc (acceptable), and LD (low discrimination). If VITEK 2 analysis resulted in low discrimination between two species and an additional Voges-Proskauer test proposed by the device could discriminate the species, the final result is in boldface.

^dThe number of isolates having discrepant pyrosequencing and VITEK 2 results at the group level.

^eThe isolates with a heterogeneous sequence in either region were identified using the other region.

^fOne isolate had heterogeneous sequences in both regions, and hence it could be identified only as Streptococcus by pyrosequencing.