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. 2007 Jan 17;45(3):897–901. doi: 10.1128/JCM.02022-06

TABLE 2.

Linearity and reproducibility of general HPV DNA real-time PCR on the Mx4000 and LightCycler

Parameter Value(s) with HPV DNA protocol
Mx4000 LightCycler
Threshold (HPV DNA copies/assay)
    50% 15 25
    95% 150 200
Linearity (log10 DNA copies/ml) 1.17-8.39 1.39-7.69
Reproducibility at 500 copies (mean CT, SD, CV [%])a
    Intra-assay 31.62, 0.58, 1.8 31.65, 0.69, 2.2
    Interassay 32.59, 0.65, 4.0 33.19, 0.70, 3.2
a

SD, standard deviation; CV, coefficient of variation.