TABLE 1.

Primers, probes, and PCR specifications used for amplification of various parts of the MIE gene^a

Primer/probe designation	Sequence, 5′→3′	Genomic coordinateb	Cycling conditions
RQ1	GACTAGTGTGATGCTGGCCAAG	2719	95°C/10 min → 50 times (95°C/15 s → 60°C/1 min)
RQ2	GCTACAATAGCCTCTTCCTCATCTG	2919
RQ probe	AGCCTGAGGTTATCAGTGTAATGAAGCGCC	2758
CMV 2-4F	GACCCTGATAATCCTGACGA	1506	95°C/10 min → 35 times (95°C/30 s → 53°C/30 s →
CMV 2-4R	ATGTGCTCCTTGATTCTATG	2073	72°C/30 s) → 72°C/7 min
exon4celyF	GAAATTCACTGGCGCCTTTA	2101	95°C/3 min → 35 times (95°C/1 min → 53°C/1 min →
exon4celyR	AGCACCATCCTCCTCTTCCT	3169	72°C/2 min) → 72°C/7 min
CMV4F	AGTGAGTTCTGTCGGGTGCT	2678	95°C/3 min → 25 times (95°C/30 s → 53°C/30 s →
CMV4R	CCCTCCTCCTCTTCCTCATC	3075	72°C/1 min) → 72°C/7 min

^aRQ1, RQ2, and RQ probe, primers and probe for Q-PCR; CMV 2-4F and CMV 2-4R, primers for qualitative PCR; exon4celyF, exon4celyR, CMV4F, and CMV4R, primers for nested PCR and consecutive sequencing.

^bMIE gene sequence (GenBank accession no. M21295).