Unique asparagine insert in G4P[8] virus from 2005 in Nicaragua. Amino acid alignment of the rotavirus VP7 gene using ClustalW, version 1.8, with default parameters. The alignment reveals an asparagine insert at position 76 in the 2005 Nicaraguan isolates, which also is present in isolates from Argentina (1996 to 1998), Uruguay (1999), and Brazil (2000 to 2004). *, conserved residue; :, position with conserved substitutions; ., position with semiconserved substitutions.