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. Author manuscript; available in PMC: 2008 Feb 15.

Published in final edited form as: Biochem Pharmacol. 2006 Oct 29;73(4):481–490. doi: 10.1016/j.bcp.2006.10.026

Fig. 2 — Inducible expression of c-Jun or TAM-67. A. MCF7-c-Jun cells were maintained in the presence (Dox+) or absence (Dox-) of doxycycline for the times indicated, and cell extracts prepared and subjected to immunoblotting with anti-c-Jun, anti-flag-tag, and anti-actin antibodies, as indicated. B. MCF7-TAM-67 cells were maintained in the presence (Dox+) or absence (Dox-) of doxycycline for the times indicated, and cell extracts prepared and subjected to immunoblotting for detection of endogenous c-Jun or TAM-67, the latter using either an antibody (sc-44) to the COOH terminus of c-Jun, or using anti-flag-tag antibody, as indicated. Actin served as a loading control.

Fig. 2 — Inducible expression of c-Jun or TAM-67. A. MCF7-c-Jun cells were maintained in the presence (Dox+) or absence (Dox-) of doxycycline for the times indicated, and cell extracts prepared and subjected to immunoblotting with anti-c-Jun, anti-flag-tag, and anti-actin antibodies, as indicated. B. MCF7-TAM-67 cells were maintained in the presence (Dox+) or absence (Dox-) of doxycycline for the times indicated, and cell extracts prepared and subjected to immunoblotting for detection of endogenous c-Jun or TAM-67, the latter using either an antibody (sc-44) to the COOH terminus of c-Jun, or using anti-flag-tag antibody, as indicated. Actin served as a loading control.