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. Author manuscript; available in PMC: 2008 Feb 15.
Published in final edited form as: Biochem Pharmacol. 2006 Oct 29;73(4):481–490. doi: 10.1016/j.bcp.2006.10.026

Fig. 4.

Fig. 4

Effect of vinblastine on c-Jun expression and phosphorylation in MCF7-TAM-67 cells. MCF7-TAM-67 cells were maintained either in the presence (top panel) or absence (lower panel) of doxycycline for 3 d and then treated with 0.3 μM vinblastine for the times indicated. Cell extracts were prepared and subjected to immunoblotting for detection of c-Jun, phospho-c-Jun, TAM-67, or actin, as indicated. The right-hand lane in the lower panel (KB3+) represents an extract from vinblastine-treated KB-3 cells as a positive control for c-Jun and phospho-c-Jun.