Figure - PMC

Skip to main content

View full-text article in PMC

. Author manuscript; available in PMC: 2008 Feb 15.

Published in final edited form as: Biochem Pharmacol. 2006 Oct 29;73(4):481–490. doi: 10.1016/j.bcp.2006.10.026

Fig. 6 — Effects of c-Jun or TAM-67 expression on vinblastine-induced cell death. A. MCF7-c-Jun cells were maintained either in the presence of doxycyline or in the absence of doxycycline for 3 d and then untreated or treated with the indicated concentration of vinblastine (VBL) for 3 d. Cell death was determined by trypan blue exclusion as described in Materials and Methods. Results represent mean ± S.D. (n = 6). The extent of cell death was highly significantly different at each vinblastine concentration in cells overexpressing c-Jun versus those not overexpressing c-Jun (* P <0.02, ** P <0.0006, and ***P <0.001, by two-tailed t test).

B. MCF7-TAM-67 cells were maintained either in the presence of doxycycline or in the absence of doxycycline for 3 d and then untreated or treated with the indicated concentration of vinblastine (VBL) for 3 d. Cell death was determined using trypan blue exclusion as described in Materials and Methods. Results represent mean ± S.D. (n = 6).

C. Effect of c-Jun expression on vinblastine-induced apoptosis. MCF7-c-Jun cells were maintained either in the presence of doxycycline or absence of doxycycline for 3 d and then untreated or treated with the indicated concentration of vinblastine (VBL) for 2 d. The extent of apoptosis, indicated as a fold-increase over the value in untreated cells, was determined using a Cell Death ELISA kit, as described in Materials and Methods. Results represent mean ± S.D. (n = 6).

Fig. 6 — Effects of c-Jun or TAM-67 expression on vinblastine-induced cell death. A. MCF7-c-Jun cells were maintained either in the presence of doxycyline or in the absence of doxycycline for 3 d and then untreated or treated with the indicated concentration of vinblastine (VBL) for 3 d. Cell death was determined by trypan blue exclusion as described in Materials and Methods. Results represent mean ± S.D. (n = 6). The extent of cell death was highly significantly different at each vinblastine concentration in cells overexpressing c-Jun versus those not overexpressing c-Jun (* P <0.02, ** P <0.0006, and ***P <0.001, by two-tailed t test).

B. MCF7-TAM-67 cells were maintained either in the presence of doxycycline or in the absence of doxycycline for 3 d and then untreated or treated with the indicated concentration of vinblastine (VBL) for 3 d. Cell death was determined using trypan blue exclusion as described in Materials and Methods. Results represent mean ± S.D. (n = 6).

C. Effect of c-Jun expression on vinblastine-induced apoptosis. MCF7-c-Jun cells were maintained either in the presence of doxycycline or absence of doxycycline for 3 d and then untreated or treated with the indicated concentration of vinblastine (VBL) for 2 d. The extent of apoptosis, indicated as a fold-increase over the value in untreated cells, was determined using a Cell Death ELISA kit, as described in Materials and Methods. Results represent mean ± S.D. (n = 6).