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. 2000 Apr 18;97(9):4677–4682. doi: 10.1073/pnas.070049097

Figure 5.

Figure 5

HuR binds the HPV16 NRE RNA. (A) EMSA with the NRE RNA probe incubated with HeLa cell nuclear (lanes 1 and 2) or cytoplasmic extract (lanes 3 and 4) in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of a mAb against HuR (19F12). The arrow indicates the supershift of the bound RNA induced by the mAb. (B) EMSA with 10 μM GST-HuR incubated with the NRE probe in the absence (lane 2) or presence (lane 3) of the HuR mAb, or with an antisense (a/s) NRE probe (lane 1). (C) GST-HuR protein (10 μM) was UV cross-linked to the NRE RNA (lane 1) and an antisense (a/s) NRE probe (lane 2). As a control, GST protein was UV cross-linked to the NRE (lane 3).