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Competition between CstF-64 and HuR for NRE RNA binding. EMSA with the NRE RNA probe incubated with 70 μM of GST-64RBD in the absence (lane 1) or presence of increased concentrations of GST-HuR: 1 μM (lane 2), 5 μM (lane 3), and 10 μM (lane 4). In lane 5, the NRE was incubated with 10 μM GST-HuR, alone. The arrow indicates the complex formed between GST-64RBD and NRE.
