Fig. 3.

Enzymatic and biological investigations of the ascorbate-dependent peroxidase activity of rat Prdx6. (A–C) Enzymatic activity was monitored by ascorbate consumption at 265 nm in incubations containing Prdx6 (0.5 nM), H₂O₂ and ascorbate at the concentrations specified in the figures. (D) The ability of rat homogenates to support the peroxidase activity of Prdx6 was investigated before and after treatment with ascorbate oxidase as described in Experimental Procedures. The 100% value means the amount of H₂O₂ consumed during 15 min at 37°C by each homogenate in the absence of ascorbate oxidase pretreatment. The bars represent the percentage of H₂O₂ consumed when homogenates were pretreated with ascorbate oxidase.