Fig. 3.

Cells expressing SidF are resistant to apoptosis induced by staurosporine. HeLa cells were transduced with adenovirus particles harboring a construct directing expression of a GFP::SidF chimera (A and B) or GFP (C and D) for 24 h before being treated with 1 μM staurosporine for 7 h. The nuclear morphology was visualized by Hoechst 33342 staining (pseudocolored in red for ease in distinguishing) (B and D). (Scale bar: 20 μm.) (E and F) Kinetic analysis of apoptotic status of the HeLa cells (E) and U937 cells (F). Transduced cells treated with 1 μM staurosporine for 3.5, 7, and 10 h were fixed, stained, and scored for the rates of apoptosis. Data shown are one representative of at least three independent experiments performed in triplicate, with at least 350 cells inspected for each sample. ∗, P < 0.05; ∗∗, P < 0.01.