Table 1.

Gene Ontology terms that were down-regulated (P < 1 × 10⁻¹¹) in KO BAT at 21°C compared with WT BAT

GO terms	No. of features
Cytoplasm	47
Mitochondrion	40
Generation of precursor metabolites and energy	20
Inner membrane	15
Oxidoreductase activity	18
Monovalent inorganic cation transporter activity	11
Primary active transporter activity	7
Sodium ion transporter activity	7
NADH dehydrogenase (ubiquinone) activity	7
Membrane-bound organelle	49
Intracellular membrane-bound organelle	49
Mitochondrial membrane	16
Oxidoreductase activity on NADH or NADPH	7
Mitochondrial inner membrane	15
Electron transport	15

No Gene Ontology (GO) terms were significantly up-regulated (P < 1 × 10⁻¹⁰). A similar result was obtained with BAT RNA isolated from mice after 5 h of acute cold exposure (data not shown).