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. Author manuscript; available in PMC: 2007 Mar 21.
Published in final edited form as: J Biol Chem. 2006 May 25;281(31):22275–22288. doi: 10.1074/jbc.M604330200

FIGURE 10.

FIGURE 10

iPLA2 transcription is down-regulated in STS-induced apoptosis. A, Western blot analysis of iPLA2 expression during STS-induced apoptosis. INS-1 and iPLA2-INS cells were treated with 1 μM STS and harvested at the times indicated. Cell lysates were prepared and analyzed by Western blot for iPLA2 and actin. B, iPLA2 activity analysis. INS-1 and iPLA2-INS cells were treated with 1 μM STS and harvested at the times indicated. Cell lysates were prepared and analyzed for iPLA2 activity. Data are the averages ± S.D. (n = 4). p < 0.05, compared with 0 h and between INS-1 and iPLA2-INS cells. C, RT-PCR analysis of iPLA2 cDNA during STS induction. INS-1 and iPLA2-INS cells were treated with 1 μM STS and harvested at the times indicated. Total RNA was isolated from each sample, and aliquots were subjected to RT-PCR analysis for iPLA2 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH), respectively.