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. Author manuscript; available in PMC: 2007 Mar 21.
Published in final edited form as: Anal Biochem. 2006 Nov 2;360(1):23–29. doi: 10.1016/j.ab.2006.10.018

Figure 5.

Figure 5

Analysis of 488-28S rRNA riboprobe binding to 28S RNA. Using a single site binding model, Area=Areamax×MassprobeKd+Massprobe, a binding curve was generated for 5 μg of total RNA hybridized to 488-28S rRNA riboprobe. This curve demonstrates the hybridization reaction reached saturation at sufficient probe concentration, with a Kd value of 33 ng (5 μg of total RNA) for the probe target sequence. Since Kd is the half-maximal concentration of target RNA sequence, 2Kd represents the amount of target sequence present at saturation, corresponding to 66 ng.