Analysis of 488-28S rRNA riboprobe binding to 28S RNA. Using a single site binding model,
, a binding curve was generated for 5 μg of total RNA hybridized to 488-28S rRNA riboprobe. This curve demonstrates the hybridization reaction reached saturation at sufficient probe concentration, with a Kd value of 33 ng (5 μg of total RNA) for the probe target sequence. Since Kd is the half-maximal concentration of target RNA sequence, 2Kd represents the amount of target sequence present at saturation, corresponding to 66 ng.