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. Author manuscript; available in PMC: 2007 Mar 21.
Published in final edited form as: Free Radic Biol Med. 2006 Nov 10;42(3):404–412. doi: 10.1016/j.freeradbiomed.2006.11.007

Figure 1.

Figure 1

Left. The kinetics of the reduction of the NR 1 and 2 (denoted on the graph) by ascorbate measured by EPR (see Materials and Methods). The solutions of the NR, 1mM, and ascorbate (2 mM, dotted lines; 20 mM, dashed lines, and 100 mM, solid lines) in 0.1 M Na-phosphate buffer, pH 7.6, 0.1 mM DTPA, were mixed under anaerobic conditions. The mixture was immediately transferred to the 50 μl capillary tube, and the peak intensity of the low-field component of the EPR spectrum was measured over time. Right. Enlarged initial part of the reduction kinetics of the NR for 20 mM ascorbate.