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. Author manuscript; available in PMC: 2007 Mar 21.
Published in final edited form as: Free Radic Biol Med. 2006 Nov 10;42(3):404–412. doi: 10.1016/j.freeradbiomed.2006.11.007

Figure 2.

Figure 2

The kinetics of the nitroxide reduction of 1 mM nitroxide 1 by 50 mM ascorbate in deaerated 0.1 M Na-phosphate buffer, pH 7.6, 0.1 mM DTPA, measured by EPR. The time-point of an addition of 10 mM hydroxylamine 1H, 15.5 min after initiation of the kinetics, is indicated by arrow. Insert: enlarged part of the kinetics around the time-point of the hydroxylamine addition. Solid line is the best fit of experimental data to equations (1) - (4) with parameters: k1 = 0.09 M−1s−1, k−1 = 1.3×103 M−1s−1, k−2 = 0.8×103 M−1s−1, k2 = 1.0×10−2 M−1s−1, k3 = 2.6×106 M−1s−1, k−3 = 1×10−2 M−1s−1, k4 = 7.6×10−4 s−1.