Figure 7.
Kinetics of the reduction of 1mM nitroxide 1 in deaerated 0.1 M Na-phosphate buffer, pH 7.6, 0.1 mM DTPA, in the presence of various concentrations of the dehydroascorbic acid, 5 mM (□) and 10 mM (■), measured by EPR as described in the Materials and Methods. Solid lines are the best fits of the experimental data to equations (4, 5) yielding the rate constant k4 = 7×10−4s−1 and k5 = (3.0±0.4)×10−3 M−1s−1.
