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. Author manuscript; available in PMC: 2008 Jan 26.
Published in final edited form as: Mol Cell. 2007 Jan 26;25(2):219–232. doi: 10.1016/j.molcel.2007.01.005

Figure 3. HDAC Activity and Caspase-Dependent C-terminal Cleavage of HDAC3 Are Required for Sorbitol-Induced c-Jun Downregulation.

Figure 3

(A–F) Immunoblotting analyses with the indicated antibodies.

A) 3Y1 cells were pretreated for 30 min with or without TSA or NaB at the concentrations shown before sorbitol treatment for 6 h.

(B) NIH3T3 cells were treated with sorbitol for the indicated time.

(C) Treatment of 3Y1 cells with or without sorbitol for 6 h was followed by immunoblotting with antibodies recognizing aa 411–428 (left) or 330–428 (right) of HDAC3.

(D) 3Y1 cells were pretreated for 30 min with or without SP600125 (25 μM) or U0126 (25 μM) before sorbitol treatment for 6 h

(E) MEK2+/+ and MEK2−/− cells were treated with or without sorbitol for 6 h.

(F) Serum-starved 3Y1 cells were treated with or without 20% serum for 6 h.