Figure 7.

Substitutions in the β′ S_NCRID enhance pausing at λP_R′. (A) Single-round in vitro transcription time-course assays using a P_R′ template and RNAP containing the indicated β′ and σ⁷⁰ proteins. +16 and +17, 16- and 17-nt RNA species, respectively, produced from the λP_R′ template; T, 194-nt terminated transcript produced from the λP_R′ template. (B) Effects of substitutions on pause capture and pause half-life (calculated from plots shown in Supplementary Figure S1). Error bars represent standard deviations from at least three separate experiments. All data were obtained and analyzed as described for Figure 4.