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. 2007 Mar 1;26(6):1681–1690. doi: 10.1038/sj.emboj.7601624

Figure 6.

Figure 6

Effect of TPNjun on the kinetics of peptide association. (A) ELRSRK*WAI (160 nM) was mixed automatically with an equal volume of peptide-deficient HLA-B*0801fos (800 nM) (○) together with a 100-fold molar excess of β2m in 20 mM Hepes and 150 mM NaCl (pH 7.5). The time course of the reaction was monitored at 20°C. The binding of ELRSRK*WAI to peptide-deficient molecules in the presence of TPNjun (600 nM) (•) is shown. (B) As described in (A) for FLRGRK*YGL: peptide-deficient HLA-B*0801fos alone (800 nM) (○) and in the presence of TPNjun (600 nM) (•). For (A) and (B), data points were collected for 2000 s.