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. 2007 Mar 1;26(6):1737–1748. doi: 10.1038/sj.emboj.7601631

Figure 1.

Figure 1

Characterization of double affinity-purified spliceosomal A complexes. Complexes were purified by a combination of tobramycin and anti-SF3a66 affinity selection, and fractionated on a glycerol gradient. RNA was isolated from the indicated fractions, analyzed by denaturing PAGE and visualized by silver staining (upper panel) or autoradiography (lower panel). RNA identities are indicated on the right. Contaminating high-molecular-weight RNA (most likely rRNA from the 40S subunit) is indicated by an asterisk. 30S and 50S correspond to migration positions of Escherichia coli ribosomal subunits in parallel gradients.