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. 2007 Mar 1;26(6):1737–1748. doi: 10.1038/sj.emboj.7601631

Figure 3.

Figure 3

Characterization of crosslinks by RNase H digestion. (A–C) Pooled gradient fractions 10–12 were UV irradiated in the presence of psoralen. Recovered RNA was incubated with RNase H in the absence (lane 1) or presence of DNA oligonucleotides against the pre-mRNA (lane 2), U1 (lane 3) or U2 snRNA (lane 4) and then analyzed by Northern blotting as described in Figure 2. A longer exposure of the upper portion of the gel is shown below in panel C. Additional minor crosslinks are discussed in the text.