Table 1.

Protein composition of double affinity-purified human A complexes

	Mol mass (kDa)	GenBank accession no.	No. 9+10	No. 11+12	A complex (Hartmuth et al)	S. cerevisiae gene	S. pombe gene
Sm proteins
B	24.6	gi∣4507125	15	20	·	SMB1	smb1
D1	13.3	gi∣5902102	1	7	·	SMD1	smd1
D2	13.5	gi∣29294624	26	32	·	SMD2	cwf9/smd2
D3	13.9	gi∣4759160	21	24	·	SMD3	smd3
E	10.8	gi∣4507129	9	25	·	SME1	sme1
F	9.7	gi∣4507131	2	7	·	SMX3	smf1
G	8.5	gi∣4507133		15	·	SMX2	smg1
U1 snRNP
U1-70K	51.6	gi∣29568103	67	67	·	SNP1	U1-70k/SPAC19A8.13
U1-A	31.3	gi∣4759156	24	38	·	MUD1	U1-A/SPBC4B4.07c
U1-C	17.4	gi∣4507127		2	·	YHC1	SPBP35G2.09
U1 snRNP-related
FBP11	48.5	gi∣88953744	10	18		PRP40	prp40
S164 (fSAP94)	100.1	gi∣4050087	10	13		SNU71	SPBC839.10
17S U2 snRNP
U2A′	28.4	gi∣50593002	31	32	·	LEA1	U2-A/SPBC1861.08c
U2B″	25.4	gi∣4507123	8	16	·	MSL1	U2-B/SPBC8D2.09c
SF3a120	88.9	gi∣5032087	53	90	·	PRP21	sap114
SF3a66	49.3	gi∣21361376	13	7	·	PRP11	sap62
SF3a60	58.5	gi∣5803167	20	21	·	PRP9	sap61
SF3b155	145.8	gi∣54112117	69	77	·	HSH155	prp10/sap155
SF3b145	100.2	gi∣55749531	37	53	·	CUS1	sap145
SF3b130	135.5	gi∣54112121	160	196	·	RSE1	prp12/sap130
SF3b49	44.4	gi∣5032069	7	8	·	HSH49	sap49
SF3b14a/p14	14.6	gi∣7706326	23	15	·		SPBC29A3.07c
SF3b14b	12.4	gi∣14249398	14	12	·	RDS3	ini1
SF3b10	10.1	gi∣13775200		2	·	YNL138W-A/RCP10/YSF3	SPBC211.05
17S U2-related
hPRP43	90.9	gi∣68509926	16	37	·	PRP43	prp43
SPF45	45.0	gi∣14249678	4	9	·
SPF30	26.7	gi∣5032113	8	10	·		spf30
U2AF65	53.5	gi∣6005926	5	8	·	MUD2	prp2/mis11/U2AF59
U2AF35	27.9	gi∣5803207	1	5	·		U2AF23/SPAP8A3.06
SPF31	31.0	gi∣7657611	1	8	·		spf31
hPRP5	117.4	gi∣41327773	27	10	·	PRP5	prp11
SR140 (fSAPa)	118.2	gi∣51492636	1	3	·		SPBC11C11.01
CHERP	100.0	gi∣21359884		2	·
PUF60	59.9	gi∣17298690	10	10
SF3b125	103.0	gi∣45446743	3	1
U5 snRNP
220K	273.7	gi∣3661610		2	·	PRP8	spp42/cwf6/prp15
200K	244.5	gi∣45861372			·	BRR2	brr2
116K	109.4	gi∣41152056		2	·	SNU114	cwf10/spef2/snu114
102K	106.9	gi∣40807485			·	PRP6	prp1
100K	95.6	gi∣41327771			·	PRP28	prp28
52K	37.6	gi∣5174409			·	LIN1 (SNU40)	SPBC83.09c
U4/U6 snRNP
90K	77.6	gi∣4758556		1		PRP3	SPAC29E6.02
U4/U6.U5 snRNP
110K	90.2	gi∣13926068			·	SNU66	snu66
SR proteins
SF2/ASF	27.8	gi∣5902076	89	69	·
9G8	27.4	gi∣72534660	15	29	·
SRp20	19.4	gi∣4506901	22	10	·
SRp30c	25.5	gi∣4506903	4	30	·
SRp40	31.3	gi∣3929378	13	25	·
SRp55	39.6	gi∣20127499	22	29	·
SRp75	56.8	gi∣21361282	11	7
SC35 (SFRS2)	25.5	gi∣47271443	6	8	·
hTra-2 beta/SFRS10	33.7	gi∣4759098	2	3
SR-related proteins
FLJ10154	32.9	gi∣48675817	1	6
SRm160	102.5	gi∣3005587	2				SPCC825.05c
SRm300	300.0	gi∣19923466	12	1		CWC21 (?)	cwf21
HnRNP
hnRNP A1	38.7	gi∣4504445	20	43	·
hnRNP A3	39.6	gi∣34740329		4	·
hnRNP AB	36.0	gi∣12803583	2	2
hnRNP A2/B1	37.4	gi∣14043072	10	17	·
hnRNP C1/C2	33.3	gi∣4758544	8	24	·
hnRNP G	42.4	gi∣56699409			·
hnRNP K	51.0	gi∣14165435			·
hnRNP Q	69.6	gi∣23397427	4	2
hnRNP R	70.9	gi∣5031755			·
hnRNP U	90.6	gi∣14141161	7	3	·
PCBP1	37.5	gi∣5453854	4	8		HEK2
E1B-AP5	95.7	gi∣21536326			·
cap binding complex
CBP20	18.0	gi∣19923387	1	9	·	CBC2	SPBC13A2.01c
CBP80	91.8	gi∣4505343	7	9	·	STO1	SPAC6G10.07
hPRP19/CDC5 complex
hPRP19	55.2	gi∣7657381		7	·	PRP19	cwf8/prp19
CDC5 (CDC5L)	92.2	gi∣11067747	1	6		CEF1	cdc5
SPF27	21.5	gi∣5031653		3		SNT309	cwf7/spf27
PRL1 (PRLG1)	57.2	gi∣4505895				PRP46	cwf1/prp5/prl1/pi024
CCAP1 (hsp73)	70.4	gi∣5729877	2	3		SSA1	ssa1
CCAP2 (hspc148, AD-002)	26.6	gi∣7705475		9		CWC15	cwf15
Catenin β-like 1 (CTNNBL1, NAP)	65.1	gi∣18644734	3	5			SPAC1952.06c
Npw38BP	70.0	gi∣7706501	4	5
Npw38	30.5	gi∣5031957	1	1
hPRP19/CDC5-related
PRCC	52.4	gi∣40807447		2
Non-snRNP proteins (previously detected)
RBM5/LUCA15	92.1	gi∣5032031	4	4	·
FLJ10839	132.8	gi∣46852388	9	7	·
ASR2B	100.0	gi∣58331218	27	56	·		SPBC725.08
p68 (DDX5)	69.2	gi∣4758138	6	21	·	DBP2	dbp2
SF4 (F23858)	72.5	gi∣33469964	16	13	·	YNL224C (?)	SPAC2G11.04 (?)
RNPC2 (CC1.3, CAPER, fSAP59)	59.4	gi∣4757926	17	15	·		rsd1
TLS/FUS	53.4	gi∣544357	7	9	·
ELAV (HuR)	36.1	gi∣38201714	2	3	·
YB-1	35.9	gi∣34098946	2	2	·
NFAR	95.4	gi∣24234750		8	·
DDX9	142.0	gi∣4503297		1	·
Non-snRNP proteins (previously not detected)
SF1	68.3	gi∣42544130	6	7		MSL5/BBP1/YSF1	bpb1/bbp/sf1
tat SF1	85.7	gi∣21361437	4	4		CUS2	uap2
CDC2L2	91.0	gi∣16357494	5	1			SPBC18H10.15
MGC2803 (fSAP18)	18.4	gi∣13128992	6	10
RBM10	103.5	gi∣12644371	9	8			SPAC17H9.04c (?)
ZNF207	50.8	gi∣4508017	1	4
BUB3	37.2	gi∣4757880	7	16		BUB3	bub3
TCERG1 (CA150)	123.9	gi∣21327715	8	11		YPR152C	dre4
NRIP2	31.3	gi∣13899327	3	6
LOC124245	104.0	gi∣31377595	3	9
NIPP1	38.5	gi∣13699256	1	5
HCNGP	33.9	gi∣9994179	4				SPAC3H1.03
PPP1CA	38.6	gi∣4506003	1			GLC7	dis2/bws1/sds1
NF45	43.0	gi∣24234747	1
p72/DDX17	80.5	gi∣3122595		2		(DBP2)	(dbp2)
Acinus (fSAP152)	151.8	gi∣7662238	6	4
Pinin	81.6	gi∣33356174	4
RNPS1	34.2	gi∣6857826		1			rnps1
Miscellaneous
p30 DBC	102.9	gi∣24432106	2
SEC31-like 2	128.7	gi∣14149696	4			SEC31	sec31/sns-B2/pi045
AGGF1	80.9	gi∣39725952		2
CIRP	18.7	gi∣4502847		2
TAFIIB	137.0	gi∣8392875		2
Proteins were identified by LC-MSMS after separation by SDS–PAGE, and the given number is the absolute number of peptides sequenced for that protein. Proteins identified previously in A complexes purified by one-step tobramycin affinity selection (Hartmuth et al, 2002) are indicated by a dot. Proteins generally accepted to be common contaminants, such as ribosomal proteins, are not shown. The calculated mass of each protein (in kDa), its accession number in the GenBank database at the NCBI and gene names of S. cerevisiae and S. pombe homologues are indicated. ?: unclear whether the indicated yeast protein is a true orthologue.