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Immunoblot of soluble antibody fragments produced using the E. coli expression vector pOPE101. 20 μL aliquots of E. coli periplasmic fractions were separated on reducing 10% SDS-PAGE, pIII was detected using mouse mAb anti-myc tag and goat anti-mouse IgG AP. Abbreviations: PE: periplasmic fraction; OS: osmotic shock preparation.
