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Size exclusion chromatography analysis of soluble antibody fragments produced using the E. coli expression vector pOPE101. 50 μg purified antibody fragments of the A scFv, or B scFabΔC format were separated on superdex 200. Calibration was done with Chymotrypsinogen (25 kDa), Ovalbumin (43 kDa), Albumin (67 kDa) and IgG (150 kDa).
