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. Author manuscript; available in PMC: 2008 Jan 1.
Published in final edited form as: J Vasc Surg. 2007 Jan;45(1):160–168. doi: 10.1016/j.jvs.2006.09.053

Figure 2. PKCδ overexpression in RASMCs, via adenoviral transfection, inhibits proliferation and migration.

Figure 2

(A) RASMCs were infected with AdNull or AdPKCδ. Forty-eight hours after infection, cells were lysed and analyzed by immunoblotting. Additional infected cells were re-seeded into 24-well plates at a density of 10,000 cells per well on day 0 and maintained in 10% FBS. Cells were counted on days 1, 2, 5 or 7. (B) Following infection with AdNull or AdPKCδ, RASMCs were serum-starved for 48 h. Basal and PDGF-BB induced DNA synthesis was measured using the 3H-thymidine incorporation. (C) RASMCs were infected with AdNull or AdPKCδ. Forty-eight hours after infection, basal and PDGF-BB (5 ng/ml) induced chemotaxis was evaluated. (n=3, *p< 0.05, compared to AdNull control).