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Alkaline Phosphatase activity (purple/blue BCIP/NBT staining) in sheep liver after crosslinking. (A) Frozen, non-crosslinked liver shows intense purple staining; TGA or Glut crosslinked liver (B&C respectively) display intense blue ALP staining; (D) reduction of alkaline phosphatase activity is evident in liver crosslinked with TGA/MABP. 100x Magnification.
