Figure 2.

IGF-1 induces RGM1 cell migration and proliferation in vitro. IGF-1 plus EGF exert additive effect on RGM1 cell migration. A: A representative photomicrograph of RGM1 cells migrating across wound edge. There is increased cell migration in response to treatment with 5, 10, and 50 ng/ml IGF-1 compared with medium only control. B: Data for cell migration studies showing a peak cell migration in the presence of 10 ng/ml IGF-1. *P value <0.001 compared with medium only control. All cell migration studies were done in triplicates and repeated twice; three random microscopic fields were selected for counting in each experiment (n = 9). C: Treatment with IGF-1 plus EGF exerts an additive effect on cell migration compared with either growth factor alone. *P value <0.05 compared with medium only control. **P value <0.05 compared with IGF-1 alone or EGF alone. D: Proliferation assay showing an increase in [³H]thymidine incorporation in RGM1 cells treated with 5, 10, and 50 ng/ml IGF-1 for 24 hours compared with medium only control. Experiments were done in triplicates and repeated twice (n = 9). E: Treatment with IGF-1 significantly increases BrdU uptake by RGM1 cells. *P value <0.05 compared with medium only control.