Table 2.
Serum, Urinary, and Histological Findings in Sham-Operated (2K) and Uninephrectomized (1K) Mice
| 2K
|
1K
|
||||
|---|---|---|---|---|---|
| Wild-type + nil (n = 7) | db/db + nil (n = 7) | db/db + nil (n = 9) | db/db + vehicle (n = 7) | db/db + BL5923 (n = 8) | |
| Renal function | |||||
| Urine albumin/creatinine | 0.1 ± 0.1 | 0.4 ± 0.1 | 0.3 ± 0.9 | 0.2 ± 0.1 | 0.2 ± 0.1 |
| BUN (mg/dl) | 27 ± 5 | 42 ± 18 | 51 ± 10 | 37 ± 6 | 37 ± 7 |
| Glomerulosclerosis score | |||||
| 0 (no lesion) | 91 ± 5 | 12 ± 6 | 0 ± 0 | 0 ± 0 | 0 ± 0 |
| 1 (1 to 24%) | 6 ± 2 | 20 ± 2 | 4 ± 5 | 3 ± 4 | 3 ± 5 |
| 2 (25 to 49%) | 3 ± 4 | 31 ± 9 | 23 ± 8 | 27 ± 4 | 29 ± 6 |
| 3 (50 to 74%) | 0 ± 0 | 26 ± 11 | 32 ± 3 | 39 ± 5 | 33 ± 4 |
| 4 (75 to 100%) | 0 ± 0 | 11 ± 3 | 40 ± 10 | 31 ± 9 | 34 ± 7 |
| Cellular response (cells/glomerulus or hpf) | |||||
| Glomerular Ki-67+ | 0.2 ± 0.1 | 0.5 ± 0.1 | 0.8 ± 0.4 | 0.9 ± 0.2 | 0.8 ± 0.3 |
| Mac-2+ | 0.2 ± 0.1 | 1.7 ± 0.1 | 2.4 ± 0.7 | 2.6 ± 0.3 | 2.2 ± 0.5 |
| Interstitial F4/80+ | 2.8 ± 0.3 | 8.4 ± 1.3 | 14.4 ± 2.1 | 14.1 ± 2.2 | 2.3 ± 1.5* |
| Ki-67+ | 0.7 ± 0.2 | 1.4 ± 0.3 | 3.9 ± 1.5 | 3.8 ± 1.2 | 0.7 ± 0.3* |
| Tubular Ki-67+ | 0.5 ± 0.2 | 1.7 ± 0.2 | 5.7 ± 1.5 | 5.0 ± 0.4 | 2.2 ± 0.5* |
| Peritubular capillaries (capillary cross sections/hpf) | |||||
| MECA-32+ | 123 ± 13 | 75 ± 11 | 81 ± 10 | 79 ± 9 | 103 ± 11* |
Values are means ± SEM, *P < 0.05 versus vehicle.