Figure 4.

Oxygenation of retinas from mice under normoxia or hypoxia. To evaluate the tissue hypoxia, pimonidazole hydrochloride (Hypoxyprobe-1) was injected into the peritoneal cavities of mice maintained under either normoxic or hypoxic conditions for 7 days, and pimonidazole hydrochloride incorporated into hypoxic cells was detected by immunostaining (a, b). Immunostaining for CD31 was performed to examine the retinal vasculature (c, d). Merged view (e, f) figured the hypoxic metabolism of the retinas from mice that had been maintained under hypoxia, especially in the areas distant from the blood vessels.