Levels of AVA in serum, transplanted hearts, and own hearts of vim/CFA-immunized mice. Time course of IgG AVA in serum of C57BL/6 mice immunized with vim/CFA and either receiving a 129/sv cardiac allograft (Tx) or not transplanted (No Tx), mean ± SD from five sera/group (A) (*P < 0.05). Western blot showing the presence of AVA in allografted 129/sv hearts (DH, B) or isografted C57BL/6 hearts (DH, C) placed in C57BL/6 mice immunized with vim/CFA (vim), and recipients’ own hearts (RH), removed at days 2, 4, 6, 8, and time of rejection. The OD % serum on the y axis represents the OD reading of the eluate from pooled hearts (either DH or RH) as a percentage of OD reading from pooled serum taken at the same time as hearts.