Figure 4.

Deposition of C3d in cardiac allografts. Photomicrograph of frozen section of 129/sv cardiac allograft placed in vim/CFA- (A) or hel/CFA- (B) immunized recipients taken at time of rejection, stained with FITC-conjugated rabbit anti-human C3d. Quantification of C3d deposits at days 2 to 8 and time of acute rejection (AR) revealed more C3d deposition in allografts placed in vimentin-immunized C57BL/6 recipients compared with allografts placed in hel-immunized recipients (C). Quantification is expressed as pixel units on the y axis. **P < 0.05 compared with the preceding time point and compared with hel/CFA recipient.