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. Author manuscript; available in PMC: 2007 Mar 22.
Published in final edited form as: Exp Cell Res. 2006 Oct 25;313(2):322–330. doi: 10.1016/j.yexcr.2006.10.014

Fig. 3.

Fig. 3

Gene expression and proliferation assays of control HEK cells and HEK cells transfected with plasmid constructs pSCR and pS2-3. A, hTERT mRNA levels showing hTERT knock down in cells transfected with pS2-3 as compared to the untransfected HEK. GAPDH was used as a loading control. B, Increased transcription of p53 and p21 in cells transfected with pS2-3 as compared to the untransfected HEK cells. GAPDH was used as a loading control. C, The graph shows linear growth of controls HEK and pSCR, while pS2-3 shows a significantly slower growth rate. Y-axis error bars represent ± standard error of the mean (SEM). * denotes statistical significance (n = 3, p < 0.05).