Gene expression and proliferation assays of SKOV, SKOVpS2-3, and SKOVpSCR. A, Reverse transcriptase PCR of RNA extracts from untransfected SKOV, SKOVpS2-3, and SKOVpSCR. SKOV and SKOVpSCR were used as controls. PCR was performed with primers specific to the gene of interest: hTERT, p21, and GAPDH. GAPDH was used as a loading control. B, No significant inhibition of growth was observed in association with hTERT knock down in SKOVpS2-3 (n = 3, p < 0.05).