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Cytokines. At 6 h postdosing, blood was drawn from each animal, processed into plasma, and analyzed for the indicated cytokine levels via ELISA (IL-6 and IL-12) or multiplex assay (IFN-γ). Experimentally determined lower limits of quantification values were 12 ng/ml for IL-12, 11.9 pg/ml for IFN-γ, and 7.8 ng/ml for IL-6. Bars indicate averages, and error bars indicate standard deviations for each group of n = 3 measurements (one for each of the three animals).
