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. 2007 Mar 22;104(14):5715–5721. doi: 10.1073/pnas.0701458104

Fig. 4.

Fig. 4.

Detection of monkey antibodies against human Tf. (A) Total antinanoparticle antibody titer was determined by ELISA on serum samples taken predosing (acclimation) and on day 18. (B) Serum samples (day 18) were incubated with human holo-Tf (1 or 100 μg/ml) or HSA (1 or 100 μg/ml) before antibody titer determination by ELISA. Incubation with HSA had a minimal effect on the ELISA signal, whereas there was a sharp dose-dependent reduction in the ELISA signal upon Tf incubation. This result supports the rationale that the antibodies generated specifically recognize the Tf moiety of the AD-PEG-Tf component of the nanoparticles.