Fig. 4.

HSCs show a coordinated reprogramming of multiple key properties. Preswitch HSCs are actively cycling and demonstrate a high rate of self-renewal after transplantation into irradiated hosts, a higher output of myeloid (GM) cells, and a distinct gene expression profile. Postswitch HSCs are predominantly quiescent and display a lower rate of self-renewal after transplantation into irradiated hosts, a lower proportional output of myeloid cells, and a different gene expression profile from preswitch HSCs.