Figure 1.
DNA binding and dimerization of the P328L329 mutant. (a) Scheme of the related human transcription factor HNF1α and HNF1β with the frameshift mutations P328L329fsdelCCTCT (P328L329) and R137-K161del. The various domains are indicated, and the numbers refer to the amino acid positions. The homology between HNF1α and HNF1β is given (2). (b) Gel-retardation assay using the 32P-labeled HNF1-binding site as a probe. The additions of the various in vitro translation products and of the antibodies are given. The heterodimers are marked with an asterisk. The methods used are described (21).