Figure 3.

Phenotypic changes in pronephros formation observed in living Xenopus larvae (stages 45–48). Two-cell-stage embryos were injected with 0.25 ng of GFP RNA together with 1 ng of RNA encoding P328L329 (a and b), HNF1β (c and d), or HNF1α (e and f). Larvae expressing green fluorescence on the left or right side are given in a, c, and e. The same animals were examined for pronephros formation at higher magnification (b, d, and f). The injected side and the pronephros are marked by black arrowheads and by red arrows, respectively. The techniques have been described (24).